Poieskin® is a Human Full Thickness Skin Model entirely produced by 3D bioprinting.
Poieskin® consists of a dermal compartment composed of primary human fibroblasts embedded in a collagen I matrix overlaid by a stratified epidermis derived from primary human keratinocytes.
Histological analyses show a remodeled dermis with neo-collagen I synthesis and an epidermis with at least 8 cell layers as well as a stratum corneum.
Poieskin® stains positive for human collagen I in the dermis, epidermal differentiation markers (cytokeratin 5, cytokeratin 10, filaggrin) and a dermo-epidermal junction marker (collagen IV).
Poieskin® biofabrication starts with dermis bioprinting and dermis maturation, followed by keratinocytes bioprinting, epidermal proliferation and stratification at the air liquid interface.
Dermis bioprinting proceeds by depositing fibroblasts and collagen layer-by-layer to give rise to a 3D cell pattern. This bioprinted pattern (number of collagen layers, distance between cells and number of cells per droplet) has been optimized to reach a controlled cell distribution within the dermis. Accordingly, Poieskin® dermal compartment results from studies dealing with fibroblasts 3D self-organization over time. (cf. video)
Following dermis maturation, keratinocytes are bioprinted on the surface of the dermal equivalent. The keratinocyte cell pattern has been designed to ensure a homogeneous cell seeding and an optimized cell proliferation phase. Finally, the skin constructs are raised to the air-liquid interface to enable epidermal stratification.
Poieskin® biofabrication benefits from the latest advances in 3D bioprinting technology. The high precision and resolution of Poietis bioprinter NGB 17.03 as well as the embedded in-line monitoring systems enable to control the quality of each bioprinted layer and hence to manufacture controlled 3D cell structures and reproducible tissue models.